Journal: EMBO molecular medicine

Article Title: Mitochondrial MsrB2 serves as a switch and transducer for mitophagy.

doi: 10.15252/emmm.201910409

Figure Lengend Snippet: Figure 1. Identification of MsrB2 as LC3 interaction protein in human platelets.

Article Snippet: Healthy/DM platelet lysates and cell lysates (after transient transfection) were mixed with the specific target antibody (1 lg of LC3 antirabbit antibody (Abcam) and 2 lg of Parkin anti-goat antibody (Abcam) or 1.5 lg of Parkin anti-rabbit antibody (Abcam) for Parkin IP; 1.5 lg of MsrB2 anti-rabbit antibody (laboratory made) for MsrB2 IP; and 1 lg of GFP anti-rabbit antibody (Abcam, USA) or GFP-Trap bead (Chromotek, USA), and the same species IgG control with HC) and incubated overnight at 4°C.

Techniques:

Journal: EMBO molecular medicine

Article Title: Mitochondrial MsrB2 serves as a switch and transducer for mitophagy.

doi: 10.15252/emmm.201910409

Figure Lengend Snippet: Figure 2. MsrB2 is needed for mitophagy.

Article Snippet: Healthy/DM platelet lysates and cell lysates (after transient transfection) were mixed with the specific target antibody (1 lg of LC3 antirabbit antibody (Abcam) and 2 lg of Parkin anti-goat antibody (Abcam) or 1.5 lg of Parkin anti-rabbit antibody (Abcam) for Parkin IP; 1.5 lg of MsrB2 anti-rabbit antibody (laboratory made) for MsrB2 IP; and 1 lg of GFP anti-rabbit antibody (Abcam, USA) or GFP-Trap bead (Chromotek, USA), and the same species IgG control with HC) and incubated overnight at 4°C.

Techniques:

Journal: EMBO molecular medicine

Article Title: Mitochondrial MsrB2 serves as a switch and transducer for mitophagy.

doi: 10.15252/emmm.201910409

Figure Lengend Snippet: Figure 5. Methionine sulfoxidation (MetO) and methionine sulfide reductase B2 (MsrB2) are increased, along with Parkin, in DM platelets.

Article Snippet: Healthy/DM platelet lysates and cell lysates (after transient transfection) were mixed with the specific target antibody (1 lg of LC3 antirabbit antibody (Abcam) and 2 lg of Parkin anti-goat antibody (Abcam) or 1.5 lg of Parkin anti-rabbit antibody (Abcam) for Parkin IP; 1.5 lg of MsrB2 anti-rabbit antibody (laboratory made) for MsrB2 IP; and 1 lg of GFP anti-rabbit antibody (Abcam, USA) or GFP-Trap bead (Chromotek, USA), and the same species IgG control with HC) and incubated overnight at 4°C.

Techniques:

Journal: EMBO molecular medicine

Article Title: Mitochondrial MsrB2 serves as a switch and transducer for mitophagy.

doi: 10.15252/emmm.201910409

Figure Lengend Snippet: Figure 6. MsrB2 is ubiquitinated by Parkin.

Article Snippet: Healthy/DM platelet lysates and cell lysates (after transient transfection) were mixed with the specific target antibody (1 lg of LC3 antirabbit antibody (Abcam) and 2 lg of Parkin anti-goat antibody (Abcam) or 1.5 lg of Parkin anti-rabbit antibody (Abcam) for Parkin IP; 1.5 lg of MsrB2 anti-rabbit antibody (laboratory made) for MsrB2 IP; and 1 lg of GFP anti-rabbit antibody (Abcam, USA) or GFP-Trap bead (Chromotek, USA), and the same species IgG control with HC) and incubated overnight at 4°C.

Techniques:

Journal: EMBO molecular medicine

Article Title: Mitochondrial MsrB2 serves as a switch and transducer for mitophagy.

doi: 10.15252/emmm.201910409

Figure Lengend Snippet: Figure 7. MsrB2 is released by mitochondrial rupture.

Article Snippet: Healthy/DM platelet lysates and cell lysates (after transient transfection) were mixed with the specific target antibody (1 lg of LC3 antirabbit antibody (Abcam) and 2 lg of Parkin anti-goat antibody (Abcam) or 1.5 lg of Parkin anti-rabbit antibody (Abcam) for Parkin IP; 1.5 lg of MsrB2 anti-rabbit antibody (laboratory made) for MsrB2 IP; and 1 lg of GFP anti-rabbit antibody (Abcam, USA) or GFP-Trap bead (Chromotek, USA), and the same species IgG control with HC) and incubated overnight at 4°C.

Techniques:

Journal: Food science and biotechnology

Article Title: Methionine strengthens anti-inflammation of rice protein via depressing NF-κB activation and stimulating Msr expression in rats fed cholesterol-enriched diets.

doi: 10.1007/s10068-022-01074-3

Figure Lengend Snippet: Fig. 3 A Protein level of MsrA. B Gene expression of MsrA. C Protein level of MsrB2. D Gene expression of MsrB2. E Protein level of MsrB3. F Gene expression of MsrB3. Data are showed as the means ± SEM (n = 6). Values with different superscript letters (a-e) are significantly different from other groups in growing rats (P \ 0.05), and values with different superscript letters (A–E) are significantly different from other groups in adult rats (P \ 0.05). Msr methionine sulfoxide reductase. C, the control group respectively fed pellets. RP, growing and adult rats respectively fed with rice protein.

Article Snippet: The primary antibodies for IjBa (Proteintech, Wuhan, China), NF-jB1 (p105/p50, Proteintech), RelA (p65, Proteintech), COX-2 (Proteintech), TNF-a (Proteintech), IL-1b (Proteintech), IL-6 (Proteintech), iNOS (Proteintech), IL-10 (Santa Cruz Biotechnology, Santa Cruz, CA, USA), MsrA (Proteintech), MsrB2 (Proteintech), MsrB3 (Proteintech), H1.2 (Proteintech), GAPDH (Proteintech) and a secondary antibody (Santa Cruz Biotechnology) were used in this study.

Techniques: Gene Expression, Control

Journal: Food science and biotechnology

Article Title: Methionine strengthens anti-inflammation of rice protein via depressing NF-κB activation and stimulating Msr expression in rats fed cholesterol-enriched diets.

doi: 10.1007/s10068-022-01074-3

Figure Lengend Snippet: Fig. 3 A Protein level of MsrA. B Gene expression of MsrA. C Protein level of MsrB2. D Gene expression of MsrB2. E Protein level of MsrB3. F Gene expression of MsrB3. Data are showed as the means ± SEM (n = 6). Values with different superscript letters (a-e) are significantly different from other groups in growing rats (P \ 0.05), and values with different superscript letters (A–E) are significantly different from other groups in adult rats (P \ 0.05). Msr methionine sulfoxide reductase. C, the control group respectively fed pellets. RP, growing and adult rats respectively fed with rice protein.

Article Snippet: The primary antibodies for IjBa (Proteintech, Wuhan, China), NF-jB1 (p105/p50, Proteintech), RelA (p65, Proteintech), COX-2 (Proteintech), TNF-a (Proteintech), IL-1b (Proteintech), IL-6 (Proteintech), iNOS (Proteintech), IL-10 (Santa Cruz Biotechnology, Santa Cruz, CA, USA), MsrA (Proteintech), MsrB2 (Proteintech), MsrB3 (Proteintech), H1.2 (Proteintech), GAPDH (Proteintech) and a secondary antibody (Santa Cruz Biotechnology) were used in this study.

Techniques: Gene Expression, Control

Journal: Food science and biotechnology

Article Title: Methionine strengthens anti-inflammation of rice protein via depressing NF-κB activation and stimulating Msr expression in rats fed cholesterol-enriched diets.

doi: 10.1007/s10068-022-01074-3

Figure Lengend Snippet: Fig. 4 The anti-inflammatory action, reflected by the downregulation of inflammatory mediators (COX-2, TNF-a, IL-1b, IL-6 and iNOS) and the upregulation of anti-inflammatory mediator (IL-10), can be induced by rice protein (RP) and enhanced by methionine (Met) in growing and adult rats fed cholesterol-enriched diets via inhibiting

Article Snippet: The primary antibodies for IjBa (Proteintech, Wuhan, China), NF-jB1 (p105/p50, Proteintech), RelA (p65, Proteintech), COX-2 (Proteintech), TNF-a (Proteintech), IL-1b (Proteintech), IL-6 (Proteintech), iNOS (Proteintech), IL-10 (Santa Cruz Biotechnology, Santa Cruz, CA, USA), MsrA (Proteintech), MsrB2 (Proteintech), MsrB3 (Proteintech), H1.2 (Proteintech), GAPDH (Proteintech) and a secondary antibody (Santa Cruz Biotechnology) were used in this study.

Techniques: